MicroFilament Analyzer, an image analysis tool for quantifying fibrillar orientation, reveals changes in microtubule organization during gravitropism
نویسندگان
چکیده
منابع مشابه
Structural analysis of human neutrophil migration: Centriole, microtubule, and microfilament orientation and function during chemotaxis
Orientation of nucleus, centriole, microtubules, and microfilaments within human neutrophils in a gradient of chemoattractant (5 percent Escherichia coli endotoxin-activated serum) was evaluated by electron microscopy. Purified neutropils (hypaque-Ficoll) were placed in the upper compartment of chemotactic chambers. Use of small pore (0.45 mum) micropore filters permitted pseudopod penetration,...
متن کاملMicrofilament Distribution in Maize Meiotic Mutants Correlates with Microtubule Organization.
Microtubules and microfilaments often codistribute in plants; their presumed interaction can be tested with drugs although it is not always clear that these are without side effects. In this study, we exploited mutants defective in meiotic cell division to investigate in a noninvasive way the relationship between the two cytoskeletal elements. By staining unfixed, permeabilized cells with rhoda...
متن کاملMicrotubule and microfilament organization in maturing human oocytes.
Various stages of immature human oocytes were imaged for microtubule, microfilament and chromatin organization. After germinal vesicle breakdown, a small microtubule aster was observed near the condensed chromatin. The asters appeared to elongate and encompass the condensed chromatin. At metaphase I stage, microtubules were detected in the meiotic spindle. The meiotic spindle in metaphase II wa...
متن کاملSIPv6 analyzer: an analysis tool for 3GPP IMS services
The 3rd Generation Partnership Project (3GPP) defines IP Multimedia Core Network Subsystem (IMS) to support IP-based multimedia services. IMS utilizes protocols such as IP version 6 (IPv6), Session Initiation Protocol (SIP), and Real-time Transport Protocol (RTP) to deliver the multimedia content. This paper proposes an analysis tool referred to as SIPv6 Analyzer to investigate the IMS-related ...
متن کاملMicrotubule Organization in the Axon: TRIM46 Determines the Orientation
The microtubule cytoskeleton is a major determinant in neuronal polarity. In this issue of Neuron, van Beuningen et al. (2015) now report that TRIM46 forms parallel microtubule bundles in the proximal axon and reveal that it is crucial for the establishment and maintenance of neuronal polarity.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Plant Journal
سال: 2013
ISSN: 0960-7412
DOI: 10.1111/tpj.12174